Tox21BodyMap provides a visualization of mapping 971 Tox21/ToxCast assay targets to regions of the human body, and allows users to create hypotheses about what some of the target tissues may be for specific chemicals. The webserver relies upon data on 9,270 chemicals with Tox21 assay activities, information on biological targets of the assays, and an approach that combines expert knowledge with tissue-specific gene expression levels to map assay targets to organ systems, providing an intuitive display of chemical bioactivity patterns. There are a variety of reasons why the mappings in this application could be inaccurate including, but not limited to, incompatibility of the chemical with in vitro test systems (e.g. volatility), activity cliffs due to limits of solubility and the concentration range used in the in vitro assays, absence of bioactivation/metabolism in the in vitro systems, and chemical-specific toxicokinetic/chemical distribution in vivo. This tool is solely a knowledge mapping and data visualization resource, and has not been rigorously evaluated for target organ predictivity. Tox21BodyMap should therefore not be used as the basis for chemical hazard characterization or risk assessment, and is solely intended for exploratory purposes and hypothesis generation.
* This website is free and open to all users and there is no login requirement.
Navigation is available for the Tox21 chemical library. The list of chemicals can be browsed on the EPA chemical dashboard and on the webserver by CASRN or name. Gene expressions by body part were extracted using the Illumina Correlation Engine (version 12-2019) and Tox21 assay results were obtained from the EPA ToxCast data, invitroDBv3.2.
Visualization includes three parts: